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- 2’-O-methyl RNA methyltransferase:
The 2’-O-methylation of ribosomal RNA is one of the most common
ways bacteria can obtain antibiotic resistance. The structure of
the 2’-O-methyl RNA methyltransferase from B. pseudomallei
(BupsA.00072.a) contains a 31 (trefoil) protein knot. According
to the protein knot server (http://knots.mit.edu/), which
confirmed that this structure has a knot, there are only 40
similar structures in the PDB, including several SpoU-like RNA
methyltransferases. The fold of these RNA methyltransferases is
quite different from the classical methyltransferase fold,
although related to the other SpoU-like RNA methyltransferases.
The ribbon diagram in the figure shows it to be a dimer and the
thread of the knot can be seen as the orange-red section passing
through the yellow-green section in the bottom dimer. Most SpoU-like
RNA methyltransferases also contain an RNA binding domain (RBD),
but this RNA methyltransferase does not contain this domain,
suggesting it may bind an accessory protein, or perhaps target a
different substrate than other enzymes of this family.
For more information, please see the
Protein Data Bank entry
3e5y.
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