Cloning & Expression Testing Team

The Cloning & Expression teams at each site will design PCR primers to amplify or synthesize the targets genes, clone them into the appropriate Vectors, and test them for soluble expression in Bacterial Expression, Cell-Free Expression, Baculovirus Expression and Mammalian Expression systems.

SSGCID personnel involved in Cloning & Expression include:

SeattleBiomed - Cell-Free Expression
Elizabeth Mundt

Emerald - Bacterial Expression and Baculovirus Expression systems
Don L:orimer (Director Molecular Biology)
Amy Raymond (Senior Scientist)

UW-PPG - Bacterial Expression system
Steve Nakazawa Hewitt (Group Manager)
Janhavi Bhandari (Res. Assoc. Scientist)