SSGCID strongly encourages submission of Target Requests by webform to speed to processing time. However, should the requestor have a large number of targets to submit, we will accept submissions by email.
Applicants should e-mail the following information to firstname.lastname@example.org. Failure to include all of the requested information may results in a delay in consideration of the target request. Please note that Our pipeline is most successful with soluble proteins or domains (no trans-membrane regions) shorter than 700 residues and containing at most 10 cysteines.
Requestor information: the applicant’s name, institution and contact information.
Target description: Please provide Uniprot ID(s) for the target(s). If no UniProt ID is available, please provide a hyperlink to the webpage describing the sequence. If there is no webpage available, please include DNA sequence and annotation.
Area of biomedical interest: Please select the most relevant.
Justification: Include a brief (one or two sentence) description of how determination of the target structure(s) will enhance research progress in the field.
Justification evidence: Describe supporting experimental evidence and/or relevant literature for functional or biological relevance of the target(s) (optional).
- If constructs are available, please provide construct name, sequence and expression information. Does the construct produce soluble protein, if so how much have you documented and under which conditions. List any important post-translational protein modifications, additives or ligands which may improve results.
- If protein is available, can you provide a minimum of 150 μl at ~5 mg/ml soluble protein to fast-track the request? If so, please provide the expressed protein sequence, vector and tag and list any additional information such as ligands or co-factors bound.
Intellectual property agreement: Please note that any structures successfully determined by the SSGCID will be submitted to the Protein Data Bank and be part of the public domain.
From our own Jan Abendroth, a user-friendly test case in which an unknown protein fragment could be determined usin… https://t.co/pIZmebDiEi
1 week, 5 days ago
Nucleoside diphosphate kinase from Borrelia burgdorferi prepared in a transition-state complex with ADP and vanadat… https://t.co/ESh4gPtH7f
3 months, 4 weeks ago