The Target Selection Team based at CIDResearch will carry out two or three rounds of target selection each year, usually resulting in batches of 100-300 protein targets. More detailed information about each batch can be found by following the links below. In addition, Target Requests from the scientific community will be reviewed for entry into the Production Pipeline on a monthly basis.
A series of bioinformatic and manual filters will be used to select targets from all the proteins predicted from the genomes of the targeted organisms. These target selection filters will include sequence similarity to known drug targets, documented or potential roles in cell growth, pathogenesis, or drug resistance, as well as markers of infection and vaccine candidates (based on their annotation and/or links to the PubMed database). Those already targeted by other Structural Genomics projects will be excluded through constant cross-reference with TargetTrack (which maintains a database of targets selected by other ongoing national and international structural genomics programs). Further prioritization will be assigned on the basis of physical properties (such as predicted solubility, appropriate size, and absence of low complexity sequence). These approaches will be used to select ~1200 targets for expression each year.
From our own Jan Abendroth, a user-friendly test case in which an unknown protein fragment could be determined usin… https://t.co/pIZmebDiEi
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Nucleoside diphosphate kinase from Borrelia burgdorferi prepared in a transition-state complex with ADP and vanadat… https://t.co/ESh4gPtH7f
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