Peer Reviewed Publications

Structural and Biophysical Characterization of the Mycobacterium tuberculosis Protein Rv0577, a Protein Associated with Neutral Red Staining of Virulent Tuberculosis Strains and Homologue of the Streptomyces coelicolor Protein KbpA
Buchko GW, Echols N, Flynn EM, Ng H-L, Stephenson S, Kim H, Myler PJ, Terwilliger TC, Alber T, Kim CY. Biochemistry (2017). 56 (30) 4015-1027.   PMID:pending.   PMCID:pending.

Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii
Long S, Brown KM, Drewry LL, Anthony B, Phan IQ, Sibley D. PLoS Pathog. (2017).   PMID:28475612.   PMCID:PMC5435356.

Structure of acid deoxyribonuclease
Varela-Ramirez A, Abendroth J, Mejia AA, Phan IQ, Lorimer D, Edwards TE, Aguilera R. Nucleic Acids Res. (2017). 45 (10) 6217-6227.   PMID:28369538.   PMCID:PMC5449587.

Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Moen S, Edwards TE, Dranow DM, Clifton MC, Sankaran B, Van Voorhis WC, Sharma A, Manoil C, Staker BL, Myler PJ, Lorimer DD. Sci Rep (2017). 7 (1) 223.   PMID:28303005.   PMCID:pending.

Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD co-factors
Haft DH, Pierce PG, Mayclin SJ, Sullivan A, Gardberg AS, Abendroth J, Begley DW, Phan IQ, Staker BL, Myler PJ, Marathias VM, Lorimer DD, Edwards TE. Sci Rep (2017). 7 41074.   PMID:28120876.   PMCID:PMC5264612.

Structural basis for the strict substrate selectivity of the mycobacterial hydrolase LipW
McKary MG, Abendroth J, Edwards TE, Johnson RJ. Biochemistry (2016). 55 (51) 7099-7111.   PMID:27936614.   PMCID:pending.

Backbone chemical shift assignments and secondary structure analysis of the U1 protein from the Bas-Congo virus
Buchko GW, Clifton MC, Wallace E, Atkins K, Myler PJ. Biomol NMR Assign (2017). 11 (1) 51-56.   PMID:27981424.   PMCID:pending.

The crystal structure of dihydrodipicolinate reductase from the human-pathogenic bacterium Bartonella henselae strain Houston-1 at 2.3 Å resolution
Cala A, Nadeau M, Abendroth J, Staker B, Reers A, Weatherhead A, Dobson R, Myler P, Hudson AO. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2016). 72 (12) 886-891.   PMID:27917836.   PMCID:pending.

Rickettsia prowazekii Methionine Aminopeptidase as a Promising Target for the Development of Antibacterial Agents
Helgren TR, Chen C, Wangtrakuldee P, Edwards TE, Staker BL, Abendroth JA, Sankaran B, Housley NA, Myler PJ, Audia J, Horn JR, Hagen TJ. Bioorg. Med. Chem. (2017). 16 ( ) S0968-0896.   PMID:28089350.   PMCID:pending.

Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1
Dubey R, Staker BL, Foe IT, Bogyo M, Myler PJ, Ngo H, Gubbels MJ. Mol. Microbiol. (2016). 103 (4) 618-634.   PMID:27859784.   PMCID: 5296235.

Biochemical and Structural Characterization of Selective Allosteric Inhibitors of the Plasmodium falciparum Drug Target, Prolyl-tRNA-synthetase
Nakazawa Hewitt S, Dranow DM, Horst B, Abendroth JA, Forte B, Hallyburton I, Jansen C, Baragana B, Choi R, Rivas K, Hulverson MA, Dumais M, Edwards TE, Lorimer DD, Gray DW, Read KD, Kirk K, Myler PJ, Wernimont A, Walpole C, Stacy R, Barrett LK, Gilbert IH, Van Voorhis WC. ACS Infect Dis (2017). 3 (1) 34-44.   PMID:27798837.   PMCID:27798837.

The BID domain of type IV secretion substrates forms a conserved four-helix bundle topped with a hook
Stanger F, de Beer T, Dranow D, Schirmer T, Phan I, Dehio C. Structure (2017). 25 (1) 203-211.   PMID:27889208.   PMCID:pending.
  -- Recommended by Faculty of 1000 : https://f1000.com/prime/727033390

Brucella melitensis methionyl-tRNA-synthetase (MetRS), a potential drug target for brucellosis
Ojo KK, Ranade RM, Zhang Z, Dranow DM, Myers J, Choi R, Nakazawa-Hewitt S, Edwards TE, Davies DR, Lorimer D, Boyle SM, Barrett LK, Buckner F, Fan E, Van Voorhis WC. PLoS ONE (2016). 11 (8) e0160350.   PMID:27500735.   PMCID:PMC4976878.

Accurate de novo design of hyperstable constrained peptides
Bhardwaj J, Mulligan VK, Bahl CD, Gilmore JM, Harvey P, Cheneval O, Buchko GW, Pulavarti SVSRK, Kaas Q, Eletsky A, Huang PS, Griesen P, Johnsen WA, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, Szyperski T, Craik DJ, Baker D. Nature (2016). 538 (7625) 329-335.   PMID:27626386.   PMCID:pending.

Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion by Neisseria meningitidis
Barnwal RP, Loh E, Godin KS, Yip J, Lavender H, Varani G, Tang C. Nucleic Acids Res. (2016).   PMID:27369378.   PMCID:pending.

The Rickettsia type IV secretion system: unrealized complexity mired by gene family expansion
Gillespie J, Phan I, Driscoll TP, Guilotte ML, Lehman SS, Rennoll-Bankert KE, Subramanian S, Beier-Sexton M, Myler PJ, Sayeedur Rahman M, Azad AF. Pathog Dis (2016). 74 (6) ftw058.   PMID:27307105.   PMCID:pending.
  -- Editors Choice : http://femspd.oxfordjournals.org/content/74/6/ftw058

Mycobacterium Cytidylate Kinase Appears to Be an Undruggable Target
Craig JC, Risler JK, Loesch K, Dong W, Subramanian S, Samudrala R, Van Voorhis WC, Baker D, Barrett LK. J Biomol Screen (2016). 21 (7) 695-700.   PMID:27146385.   PMCID:pending.

Cloning, expression, purification, crystallization and X-ray diffraction analysis of dihydrodipicolinate synthase from the human pathogenic bacterium Bartonella henselae strain Houston-1 at 2.1 Å resolution
Naqvi KF, Staker BL, Dobson RCJ, Serbzhinisky D, Sankaran B, Myler PJ, Hudson AO. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2015). 1 (72) 2-9.   PMID:26750477.   PMCID:PMC4708043.

Structural Insight into How Bacteria Prevent Interference between Multiple Divergent Type IV Secretion Systems.
Gillespie J, Phan IQ, Scheib H, Subramanian S, Edwards TE, Lehman SS, Piitulainen H, Sayeedur Rahman M, Rennoll-Bankert KE, Staker BL, Taira S, Stacy R, Myler PJ, Azad AF, Pulliainen A. MBio (2015). 6 (6) e01867-15.   PMID:26646013.   PMCID:PMC4676284.
  -- Recommended by Faculty of 1000 : http://f1000.com/prime/726000545

Synthesis and biological evaluation of pyrazolopyrimidines as potential antibacterial agents.
Goshu GM, Ghose D, Bain JM, Pierce PG, Begley DW, Hewitt SN, Udell HS, Myler PJ, Meganathan R, Hagen TJ. Bioorg. Med. Chem. Lett. (2015). Dec 15 (24) 5699-5704.   PMID:26584881.   PMCID:PMC4764408.

An O-Methyltransferase Is Required for Infection of Tick Cells by Anaplasma phagocytophilum.
Olivia Chavez AS, Fairman JW, Felsheim RF, Nelson CM, Herron MJ, Higgins L, Burkhardt NY, Oliver JD, Markowski TW, Kurtti TJ, Edwards TE, Munderloh UG. PLoS Pathog. (2015). 11 (11) e1005248.   PMID:26544981.   PMCID:PMC4636158.

Recent contributions of structure-based drug design to the development of antibacterial compounds
Staker BL, Buchko GW, Myler PJ. Curr. Opin. Microbiol. (2015). 27 133-8.   PMID:26458180.   PMCID:PMC4659754.

Advances in Bacterial Methionine Aminopeptidase Inhibition.
Helgren TR, Wangtrakuldee P, Staker BL, Hagen TJ. Curr Top Med Chem (2015). 16 (4) 397-414.   PMID:26268344.   PMCID:pending.

Structures of a histidine triad family protein from Entamoeba histolytica bound to sulfate, AMP and GMP.
Lorimer DD, Choi R, Abramov A, Nakazawa Hewitt S, Gardberg AS, Van Voorhis WC, Staker BL, Myler PJ, Edwards TE. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2015). 71 (Pt 5) 572-6.   PMID:25945711.   PMCID:PMC4427167.

Backbone chemical shift assignments for the sensor domain of the Burkholderia pseudomallei histidine kinase RisS: "missing" resonances at the dimer interface.
Buchko GW, Edwards TE, Hewitt SN, Phan IQ, Van Voorhis WC, Miller SI, Myler PJ. Biomol NMR Assign (2015). 9 (2) 381-5.   PMID:25957069.   PMCID:PMC4569509.

Iron superoxide dismutases in eukaryotic pathogens: new insights from Apicomplexa and Trypanosoma structures.
Phan IQ, Davies DR, Moretti NS, Shanmugam D, Cestari I, Anupama A, Fairman JW, Edwards TE, Stuart K, Schenkman S, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2015). 71 (Pt 5) 615-21.   PMID:25961325.   PMCID:PMC4427173.

Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.
Moen SO, Fairman JW, Barnes SR, Sullivan A, Nakazawa-Hewitt S, Van Voorhis WC, Staker BL, Lorimer DD, Myler PJ, Edwards TE. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2015). 71 (Pt 5) 609-14.   PMID:25945716.   PMCID:PMC4361283.

Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.
Edwards TE, Baugh L, Bullen J, Baydo RO, Witte P, Thompkins K, Phan IQ, Abendroth J, Clifton MC, Sankaran B, Van Voorhis WC, Myler PJ, Staker BL, Grundner C, Lorimer DD. J. Struct. Funct. Genomics (2015). 16 (2) 91-9.   PMID:25832174.   PMCID:PMC4631608.

Structure of an ADP-ribosylation factor, ARF1, from Entamoeba histolytica bound to Mg(2+)-GDP.
Serbzhinskiy DA, Clifton MC, Sankaran B, Staker BL, Edwards TE, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2015). 71 (Pt 5) 594-9.   PMID:25945714.   PMCID:PMC4427170.

High-resolution Crystal Structure of Dimeric VP40 From Sudan ebolavirus.
Clifton MC, Bruhn JF, Atkins K, Webb TL, Baydo RO, Raymond A, Lorimer DD, Edwards TE, Myler PJ, Saphire EO. J. Infect. Dis. (2015). 212 Suppl 2 S167-71.   PMID:25957961.   PMCID:PMC4564533.

Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Abendroth J, Choi R, Wall A, Clifton MC, Lukacs CM, Staker BL, van Voorhis W, Myler P, Lorimer DD, Edwards TE. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2015). 71 (Pt 5) 566-71.   PMID:25945710.   PMCID:PMC4427166.

Structure of uridine diphosphate N-acetylglucosamine pyrophosphorylase from Entamoeba histolytica.
Edwards TE, Gardberg AS, Phan IQ, Zhang Y, Staker BL, Myler PJ, Lorimer DD. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2015). 71 (Pt 5) 560-5.   PMID:25945709.   PMCID:PMC4427165.

Targeting Influenza A Virus RNA Promoter
Bottini A, De SK, Wu B, Varani G, Pellecchia M, Tang C. Chem Biol Drug Des (2015). 86 (4) 663-73.   PMID:25676805.   PMCID:PMC4531110.

Structure of a CutA1 divalent-cation tolerance protein from Cryptosporidium parvum, the protozoal parasite responsible for cryptosporidiosis.
Buchko GW, Abendroth J, Clifton MC, Robinson H, Zhang Y, Hewitt SN, Staker BL, Edwards TE, Van Voorhis WC, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2014). 71 (Pt 5) 522-30.   PMID:25945704.   PMCID:PMC4427160.

Solution-state NMR structure of the putative morphogene protein BolA (PFE0790c) from Plasmodium falciparum.
Buchko GW, Yee A, Semesi A, Myler PJ, Arrowsmith CH, Hui R. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2014). 71 (Pt 5) 514-21.   PMID:25945703.   PMCID:PMC4427159.

Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ. Tuberculosis (Edinb) (2014). 95 (2) 142-8.   PMID:25613812.   PMCID:PMC4361283.
  -- Author presentation of results : http://www.tuberculosisjournal.com/article/S1472-9792(14)20565-8/abstract
  -- Explore the Mtb-NTM-PDB network : https://www.ssgcid.org/publication/mtb-ntm-pdb-network/

Tyrosine aminotransferase from Leishmania infantum: A new drug target candidate.
Moreno MA, Alonso A, Alcolea PJ, Abramov A, de Lacoba MG, Abendroth J, Zhang S, Edwards T, Lorimer D, Myler PJ, Larraga V. Int. J. Parasitol. (2014). 4 (3) 347-54.   PMID:25516846.   PMCID:PMC4266777.

Structural analysis of H1N1 and H7N9 influenza A virus PA in the absence of PB1.
Moen SO, Abendroth J, Fairman JW, Baydo RO, Bullen J, Kirkwood JL, Barnes SR, Raymond AC, Begley DW, Henkel G, McCormack K, Tam VC, Phan I, Staker BL, Stacy R, Myler PJ, Lorimer D, Edwards TE. Sci Rep (2014). 4 (4) 5944.   PMID:25089892.   PMCID:PMC4123200.

Structure of tyrosine aminotransferase from Leishmania infantum.
Moreno MA, Abramov A, Abendroth J, Alonso A, Zhang S, Alcolea PJ, Edwards T, Lorimer D, Myler PJ, Larraga V. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2014). 70 (Pt 5) 583-7.   PMID:24817714.   PMCID:PMC4014323.

Structure of the Reston ebolavirus VP30 C-terminal domain.
Clifton MC, Kirchdoerfer RN, Atkins K, Abendroth J, Raymond A, Grice R, Barnes S, Moen S, Lorimer D, Edwards TE, Myler PJ, Saphire EO. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2014). 70 (Pt 4) 457-60.   PMID:24699737.   PMCID:PMC3976061.

Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase.
Linford AS, Jiang NM, Edwards TE, Sherman NE, Van Voorhis WC, Stewart LJ, Myler PJ, Staker BL, Petri WA. Mol. Biochem. Parasitol. (2014). 193 (1) 33-44.   PMID:24548880.   PMCID:PMC4022148.

A structural biology approach enables the development of antimicrobials targeting bacterial immunophilins.
Begley DW, Fox D, Jenner D, Juli C, Pierce PG, Abendroth J, Muruthi M, Safford K, Anderson V, Atkins K, Barnes SR, Moen SO, Raymond AC, Stacy R, Myler PJ, Staker BL, Harmer NJ, Norville IH, Holzgrabe U, Sarkar-Tyson M, Edwards TE, Lorimer DD. Antimicrob. Agents Chemother. (2013). 58 (3) 1458-67.   PMID:24366729.   PMCID:PMC3957879.

Solution structure of a putative FKBP-type peptidyl-propyl cis-trans isomerase from Giardia lamblia.
Buchko GW, Hewitt SN, Van Voorhis WC, Myler PJ. J. Biomol. NMR (2013). 57 (4) 369-74.   PMID:24293257.   PMCID:PMC3903135.

Mycobacterium tuberculosis Rv2179c protein establishes a new exoribonuclease family with broad phylogenetic distribution.
Abendroth J, Ollodart A, Andrews ES, Myler PJ, Staker BL, Edwards TE, Arcus VL, Grundner C. J. Biol. Chem. (2013). 289 (4) 2139-47.   PMID:24311791.   PMCID:PMC3900960.

A novel small-molecule binds to the influenza A virus RNA promoter and inhibits viral replication.
Lee MK, Bottini A, Kim M, Bardaro MF, Zhang Z, Pellecchia M, Choi BS, Varani G. Chem. Commun. (Camb.) (2013). 50 (3) 368-70.   PMID:24247110.   PMCID:PMC3894927.

Cytidine derivatives as IspF inhibitors of Burkolderia pseudomallei.
Zhang Z, Jakkaraju S, Blain J, Gogol K, Zhao L, Hartley RC, Karlsson CA, Staker BL, Edwards TE, Stewart LJ, Myler PJ, Clare M, Begley DW, Horn JR, Hagen TJ. Bioorg. Med. Chem. Lett. (2013). 23 (24) 6860-3.   PMID:24157367.   PMCID:PMC3874807.

The impact of nitric oxide toxicity on the evolution of the glutathione transferase superfamily: a proposal for an evolutionary driving force.
Bocedi A, Fabrini R, Farrotti A, Stella L, Ketterman AJ, Pedersen JZ, Allocati N, Lau PC, Grosse S, Eltis LD, Ruzzini A, Edwards TE, Morici L, Del Grosso E, Guidoni L, Bovi D, Lo Bello M, Federici G, Parker MW, Board PG, Ricci G. J. Biol. Chem. (2013). 288 (34) 24936-47.   PMID:23828197.   PMCID:PMC3750189.

Discovery of Inhibitors of Burkholderia pseudomallei Methionine Aminopeptidase with Antibacterial Activity.
Wangtrakuldee P, Byrd MS, Campos CG, Henderson MW, Zhang Z, Clare M, Masoudi A, Myler PJ, Horn JR, Cotter PA, Hagen TJ. ACS Med Chem Lett (2013). 4 (8) 669-703.   PMID:24376907.   PMCID:PMC3871185.

Crystal structure of a macrophage migration inhibitory factor from Giardia lamblia.
Buchko GW, Abendroth J, Robinson H, Zhang Y, Hewitt SN, Edwards TE, Van Voorhis WC, Myler PJ. J. Struct. Funct. Genomics (2013). 14 (2) 47-57.   PMID:23709284.   PMCID:PMC3826599.

Structural insights into the functional role of the Hcn sub-domain of the receptor-binding domain of the botulinum neurotoxin mosaic serotype C/D.
Zhang Y, Gardberg AS, Edwards TE, Sankaran B, Robinson H, Varnum SM, Buchko GW. Biochimie (2013). 95 (7) 1379-85.   PMID:23523511.   PMCID:PMC3674139.

Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh L, Gallagher LA, Patrapuvich R, Clifton MC, Gardberg AS, Edwards TE, Armour B, Begley DW, Dieterich SH, Dranow DM, Abendroth J, Fairman JW, Fox D, Staker BL, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R, Myler PJ, Stewart LJ, Manoil C, Van Voorhis WC. PLoS ONE (2012). 8 (1) e53851.   PMID:23382856.   PMCID:PMC3561365.
  -- Recommended by Faculty of 1000 : http://f1000.com/prime/717977162

Learning virulent proteins from integrated query networks.
Cadag E, Tarczy-Hornoch P, Myler PJ. BMC Bioinformatics (2012). 13 321.   PMID:23198735.   PMCID:PMC3560104.

Stabilizing additives added during cell lysis aid in the solubilization of recombinant proteins.
Leibly DJ, Nguyen TN, Kao LT, Hewitt SN, Barrett LK, Van Voorhis WC. PLoS ONE (2012). 7 (12) e52482.   PMID:23285060.   PMCID:PMC3527557.
  -- Recommended by Faculty of 1000 : http://f1000.com/prime/717977195#citeEvaluationContent-793470720

Multi-target parallel processing approach for gene-to-structure determination of the influenza polymerase PB2 subunit.
Armour BL, Barnes SR, Moen SO, Smith E, Raymond AC, Fairman JW, Stewart LJ, Staker BL, Begley DW, Edwards TE, Lorimer DD. J Vis Exp (2013). Jun 28 (76)   PMID:23851357.   PMCID:PMC3747311.

Siderocalin/Lcn2/NGAL/24p3 does not drive apoptosis through gentisic acid mediated iron withdrawal in hematopoietic cell lines.
Correnti C, Richardson V, Sia AK, Bandaranayake AD, Ruiz M, Suryo Rahmanto Y, Kovacevic Ž, Clifton MC, Holmes MA, Kaiser BK, Barasch J, Raymond KN, Richardson DR, Strong RK. PLoS ONE (2012). 7 (8) e43696.   PMID:22928018.   PMCID:PMC3424236.

Mycobacterium thermoresistibile as a source of thermostable orthologs of Mycobacterium tuberculosis proteins.
Edwards TE, Liao R, Phan I, Myler PJ, Grundner C. Protein Sci. (2012). 21 (7) 1093-6.   PMID:22544630.   PMCID:PMC3403447.

Structural characterization of a ribose-5-phosphate isomerase B from the pathogenic fungus Coccidioides immitis.
Edwards TE, Abramov AB, Smith ER, Baydo RO, Leonard JT, Leibly DJ, Thompkins KB, Clifton MC, Gardberg AS, Staker BL, Van Voorhis WC, Myler PJ, Stewart LJ. BMC Struct. Biol. (2011). 11 39.   PMID:21995815.   PMCID:PMC3212906.

Structural basis of the substrate specificity of bifunctional isocitrate dehydrogenase kinase/phosphatase.
Yates SP, Edwards TE, Bryan CM, Stein AJ, Van Voorhis WC, Myler PJ, Stewart LJ, Zheng J, Jia Z. Biochemistry (2011). 50 (38) 8103-6.   PMID:21870819.   PMCID:PMC3354702.

Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline.
Guild K, Zhang Y, Stacy R, Mundt E, Benbow S, Green A, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1027-31.   PMID:21904045.   PMCID:PMC3169397.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of aldose reductase from Giardia lamblia.
Ferrell M, Abendroth J, Zhang Y, Sankaran B, Edwards TE, Staker BL, Van Voorhis WC, Stewart LJ, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1113-7.   PMID:21904059.   PMCID:PMC3169411.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of 3-ketoacyl-(acyl-carrier-protein) reductase from Rickettsia prowazekii at 2.25 Å resolution.
Subramanian S, Abendroth J, Phan IQ, Olsen C, Staker BL, Napuli A, Van Voorhis WC, Stacy R, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1118-22.   PMID:21904060.   PMCID:PMC3169412.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

An ensemble of structures of Burkholderia pseudomallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase.
Davies DR, Staker BL, Abendroth JA, Edwards TE, Hartley R, Leonard J, Kim H, Rychel AL, Hewitt SN, Myler PJ, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1044-50.   PMID:21904048.   PMCID:PMC3169400.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of the cystathionine γ-synthase MetB from Mycobacterium ulcerans.
Clifton MC, Abendroth J, Edwards TE, Leibly DJ, Gillespie AK, Ferrell M, Dieterich SH, Exley I, Staker BL, Myler PJ, Van Voorhis WC, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1154-8.   PMID:21904066.   PMCID:PMC3169418.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structural genomics of infectious disease drug targets: the SSGCID.
Stacy R, Begley DW, Phan I, Staker BL, Van Voorhis WC, Varani G, Buchko GW, Stewart LJ, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 979-84.   PMID:21904037.   PMCID:PMC3169389.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

The Protein Maker: an automated system for high-throughput parallel purification.
Smith ER, Begley DW, Anderson V, Raymond AC, Haffner TE, Robinson JI, Edwards TE, Duncan N, Gerdts CJ, Mixon MB, Nollert P, Staker BL, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1015-21.   PMID:21904043.   PMCID:PMC3169395.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Inhibitor-bound complexes of dihydrofolate reductase-thymidylate synthase from Babesia bovis
Begley DW, Edwards TE, Raymond AC, Smith ER, Hartley RC, Abendroth J, Sankaran B, Lorimer DD, Myler PJ, Staker BL, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1070-7.   PMID:21904052.   PMCID:PMC3169404.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.
Christensen J, Gerdts CJ, Clifton MC, Stewart L. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1022-6.   PMID:21904044.   PMCID:PMC3169396.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Gene Composer in a structural genomics environment.
Lorimer D, Raymond A, Mixon M, Burgin A, Staker B, Stewart L. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 985-91.   PMID:21904038.   PMCID:PMC3169390.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of fumarate hydratase from Rickettsia prowazekii, the agent of typhus and suspected relative of the mitochondria.
Phan I, Subramanian S, Olsen C, Edwards TE, Guo W, Zhang Y, Van Voorhis WC, Stewart LJ, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1123-8.   PMID:21904061.   PMCID:PMC3169413.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Gene design, cloning and protein-expression methods for high-value targets at the Seattle Structural Genomics Center for Infectious Disease.
Raymond A, Haffner T, Ng N, Lorimer D, Staker B, Stewart L. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 992-7.   PMID:21904039.   PMCID:PMC3169391.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Chemical shift assignments for Rv0577, a putative glyoxylase associated with virulence from Mycobacterium tuberculosis
Buchko GW, Kim H, Myler PJ, Terwilliger TC, Kim CY. Biomol NMR Assign (2011). 6 (1) 43-6.   PMID:21761124.   PMCID:PMC3374633.

Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.
Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1006-9.   PMID:21904041.   PMCID:PMC3169393.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of fructose bisphosphate aldolase from Encephalitozoon cuniculi.
Gardberg A, Sankaran B, Davies D, Bhandari J, Staker B, Stewart L. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1055-9.   PMID:21904050.   PMCID:PMC3169402.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of triosephosphate isomerase from Cryptosporidium parvum.
Nguyen TN, Abendroth J, Leibly DJ, Le KP, Guo W, Kelley A, Stewart L, Myler PJ, Van Voorhis WC. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1095-9.   PMID:21904056.   PMCID:PMC3169408.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of fructose bisphosphate aldolase from Bartonella henselae bound to fructose 1, 6-bisphosphate
Gardberg A, Abendroth J, Bhandari J, Sankaran B, Staker B. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1051-4.   PMID:21904049.   PMCID:PMC3169401.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

The structure of a Burkholderia pseudomallei immunophilin-inhibitor complex reveals new approaches to antimicrobial development.
Norville IH, O'Shea K, Sarkar-Tyson M, Zheng S, Titball RW, Varani G, Harmer NJ. Biochem. J. (2011). 437 (3) 413-22.   PMID:21574961.   PMCID:PMC4045482.

High-throughput protein production and purification at the Seattle Structural Genomics Center for Infectious Disease.
Bryan CM, Bhandari J, Napuli AJ, Leibly DJ, Choi R, Kelley A, Van Voorhis WC, Edwards TE, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1010-4.   PMID:21904042.   PMCID:PMC3169394.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of a cyclin-dependent kinase from Giardia lamblia.
Leibly DJ, Newling PA, Abendroth J, Guo W, Kelley A, Stewart LJ, van Voorhis W. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1084-9.   PMID:21904054.   PMCID:PMC3169406.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success.
Choi R, Kelley A, Leibly D, Hewitt SN, Napuli A, van Voorhis W. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 998-1005.   PMID:21904040.   PMCID:PMC3169392.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of thymidylate kinase from Ehrlichia chaffeensis.
Leibly DJ, Abendroth J, Bryan CM, Sankaran B, Kelley A, Barrett LK, Stewart L, Van Voorhis WC. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1090-4.   PMID:21904055.   PMCID:PMC3169407.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of nitrilotriacetate monooxygenase component B from Mycobacterium thermoresistibile.
Zhang Y, Edwards TE, Begley DW, Abramov A, Thompkins KB, Ferrell M, Guo WJ, Phan I, Olsen C, Napuli A, Sankaran B, Stacy R, Van Voorhis WC, Stewart LJ, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1100-5.   PMID:21904057.   PMCID:PMC3169409.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Comparative analysis of glutaredoxin domains from bacterial opportunistic pathogens.
Leeper T, Zhang S, Van Voorhis WC, Myler PJ, Varani G. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1141-7.   PMID:21904064.   PMCID:PMC3169416.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structure of a Nudix hydrolase (MutT) in the Mg(2+)-bound state from Bartonella henselae, the bacterium responsible for cat scratch fever.
Buchko GW, Edwards TE, Abendroth J, Arakaki TL, Law L, Napuli AJ, Hewitt SN, Van Voorhis WC, Stewart LJ, Staker BL, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1078-83.   PMID:21904053.   PMCID:PMC3169405.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

BrabA.11339.a: anomalous diffraction and ligand binding guide towards the elucidation of the function of a 'putative β-lactamase-like protein' from Brucella melitensis.
Abendroth J, Sankaran B, Edwards TE, Gardberg AS, Dieterich S, Bhandari J, Napuli AJ, Van Voorhis WC, Staker BL, Myler PJ, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1106-12.   PMID:21904058.   PMCID:PMC3169410.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structures of a putative ζ-class glutathione S-transferase from the pathogenic fungus Coccidioides immitis.
Edwards TE, Bryan CM, Leibly DJ, Dieterich SH, Abendroth J, Sankaran B, Sivam D, Staker BL, Van Voorhis WC, Myler PJ, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1038-43.   PMID:21904047.   PMCID:PMC3169399.

Crystal structure of Toxoplasma gondii porphobilinogen synthase: insights on octameric structure and porphobilinogen formation.
Jaffe EK, Shanmugam D, Gardberg A, Dieterich S, Sankaran B, Stewart LJ, Myler PJ, Roos DS. J. Biol. Chem. (2011). 286 (17) 15298-307.   PMID:21383008.   PMCID:PMC3083160.

SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Gardberg AS, Robinson JI, Christensen JS, Staker BL, Myler PJ, Stewart LJ, Edwards TE. J. Struct. Funct. Genomics (2011). 12 (2) 83-95.   PMID:21359836.   PMCID:PMC3123459.

Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis.
Buchko GW, Hewitt SN, Napuli AJ, Van Voorhis WC, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1129-36.   PMID:21904062.   PMCID:PMC3169414.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Leveraging structure determination with fragment screening for infectious disease drug targets: MECP synthase from Burkholderia pseudomallei.
Begley DW, Hartley RC, Davies DR, Edwards TE, Leonard JT, Abendroth J, Burris CA, Bhandari J, Myler PJ, Staker BL, Stewart LJ. J. Struct. Funct. Genomics (2011). 12 (2) 63-76.   PMID:21359640.   PMCID:PMC3123455.

NMR structure of an acyl-carrier protein from Borrelia burgdorferi.
Barnwal RP, Van Voorhis WC, Varani G. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1137-40.   PMID:21904063.   PMCID:PMC3169415.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Structures of phosphopantetheine adenylyltransferase from Burkholderia pseudomallei.
Edwards TE, Leibly DJ, Bhandari J, Statnekov JB, Phan I, Dieterich SH, Abendroth J, Staker BL, Van Voorhis WC, Myler PJ, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1032-7.   PMID:21904046.   PMCID:PMC3169398.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Solution-state NMR structure and biophysical characterization of zinc-substituted rubredoxin B (Rv3250c) from Mycobacterium tuberculosis.
Buchko GW, Hewitt SN, Napuli AJ, Van Voorhis WC, Myler PJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2011). 67 (Pt 9) 1148-53.   PMID:21904065.   PMCID:PMC3169417.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Inaugural structure from the DUF3349 superfamily of proteins, Mycobacterium tuberculosis Rv0543c.
Buchko GW, Phan I, Myler PJ, Terwilliger TC, Kim CY. Arch. Biochem. Biophys. (2010). 506 (2) 150-6.   PMID:21144816.   PMCID:PMC3035944.

Crystal structure of the receptor binding domain of the botulinum C-D mosaic neurotoxin reveals potential roles of lysines 1118 and 1136 in membrane interactions.
Zhang Y, Buchko GW, Qin L, Robinson H, Varnum SM. Biochem. Biophys. Res. Commun. (2010). 404 (1) 407-12.   PMID:21130733.   PMCID:PMC3019264.

Design and initial characterization of the SC-200 proteomics standard mixture.
Bauman A, Higdon R, Rapson S, Loiue B, Hogan J, Stacy R, Napuli A, Guo W, van Voorhis W, Roach J, Lu V, Landorf E, Stewart E, Kolker N, Collart F, Myler P, van Belle G, Kolker E. OMICS (2010). 15 (1-2) 73-82.   PMID:21250827.   PMCID:PMC3110723.

Structure of a Burkholderia pseudomallei trimeric autotransporter adhesin head.
Edwards TE, Phan I, Abendroth J, Dieterich SH, Masoudi A, Guo W, Hewitt SN, Kelley A, Leibly D, Brittnacher MJ, Staker BL, Miller SI, Van Voorhis WC, Myler PJ, Stewart LJ. PLoS ONE (2010). 5 (9) e12803.   PMID:20862217.   PMCID:PMC2942831.

Nanovolume optimization of protein crystal growth using the microcapillary protein crystallization system.
Gerdts CJ, Stahl GL, Napuli A, Staker B, Abendroth J, Edwards TE, Myler P, van Voorhis W, Nollert P, Stewart LJ. J Appl Crystallogr (2010). 43 (Pt 5) 1078-1083.   PMID:22477778.   PMCID:PMC3263815.

Biological and structural characterization of a host-adapting amino acid in influenza virus.
Yamada S, Hatta M, Staker BL, Watanabe S, Imai M, Shinya K, Sakai-Tagawa Y, Ito M, Ozawa M, Watanabe T, Sakabe S, Li C, Kim JH, Myler PJ, Phan I, Raymond A, Smith E, Stacy R, Nidom CA, Lank SM, Wiseman RW, Bimber BN, O'Connor DH, Neumann G, Stewart LJ, Kawaoka Y. PLoS Pathog. (2010). 6 (8) e1001034.   PMID:20700447.   PMCID:PMC2916879.

Probing conformational states of glutaryl-CoA dehydrogenase by fragment screening.
Begley DW, Davies DR, Hartley RC, Hewitt SN, Rychel AL, Myler PJ, Van Voorhis WC, Staker BL, Stewart LJ. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2010). 67 (Pt 9) 1060-9.   PMID:21904051.   PMCID:PMC3169403.
  -- Complete SSGCID Special Issue : http://journals.iucr.org/f/issues/2011/09/00/index.html

Solution structure of Rv2377c-founding member of the MbtH-like protein family.
Buchko GW, Kim CY, Terwilliger TC, Myler PJ. Tuberculosis (Edinb) (2010). 90 (4) 245-51.   PMID:20434955.   PMCID:PMC2910232.

Structural characterization of Burkholderia pseudomallei adenylate kinase (Adk): profound asymmetry in the crystal structure of the ''open'' state.
Buchko GW, Robinson H, Abendroth J, Staker BL, Myler PJ. Biochem. Biophys. Res. Commun. (2010). 394 (4) 1012-7.   PMID:20331978.   PMCID:PMC2856777.

X-ray structure determination of the glycine cleavage system protein H of Mycobacterium tuberculosis using an inverse Compton synchrotron X-ray source.
Abendroth J, McCormick MS, Edwards TE, Staker B, Loewen R, Gifford M, Rifkin J, Mayer C, Guo W, Zhang Y, Myler P, Kelley A, Analau E, Hewitt SN, Napuli AJ, Kuhn P, Ruth RD, Stewart LJ. J. Struct. Funct. Genomics (2010). 11 (1) 91-100.   PMID:20364333.   PMCID:PMC2939714.

The role of medical structural genomics in discovering new drugs for infectious diseases.
Van Voorhis WC, Hol WG, Myler PJ, Stewart LJ. PLoS Comput. Biol. (2009). 5 (10) e1000530.   PMID:19855826.   PMCID:PMC2756625.

Backbone and side chain (1)H, (13)C, and (15)N NMR assignments for the organic hydroperoxide resistance protein (Ohr) from Burkholderia pseudomallei.
Buchko GW, Hewitt SN, Napuli AJ, Van Voorhis WC, Myler PJ. Biomol NMR Assign (2009). 3 (2) 163-6.   PMID:19888681.   PMCID:PMC2774895.

Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer.
Raymond A, Lovell S, Lorimer D, Walchli J, Mixon M, Wallace E, Thompkins K, Archer K, Burgin A, Stewart L. BMC Biotechnol. (2009). 9 (Apr 21) 37.   PMID:19383143.   PMCID:PMC2680836.

The Seattle Structural Genomics Center for Infectious Disease (SSGCID).
Myler PJ, Stacy R, Stewart L, Staker BL, Van Voorhis WC, Varani G, Buchko GW. Infect Disord Drug Targets (2009). 9 (5) 493-506.   PMID:19594426.   PMCID:PMC2857597.

The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS).
Gerdts CJ, Elliott M, Lovell S, Mixon MB, Napuli AJ, Staker BL, Nollert P, Stewart L. Acta Crystallogr. D Biol. Crystallogr. (2008). 64 (Pt 11) 1116-22.   PMID:19020349.   PMCID:PMC2585160.